Facilitation of RNA enzyme activity in the molecular crowding media of cosolutes.

@article{Nakano2009FacilitationOR,
  title={Facilitation of RNA enzyme activity in the molecular crowding media of cosolutes.},
  author={Shu-Ichi Nakano and Hisae Tateishi Karimata and Yuichi Kitagawa and Naoki Sugimoto},
  journal={Journal of the American Chemical Society},
  year={2009},
  volume={131 46},
  pages={16881-8}
}
Short RNA sequences exhibiting the activity of a target RNA cleavage are promising for cellular gene regulation and biosensor research, but the reaction media different from an aqueous solution may cause unanticipated molecular interactions and properties. In this study, we investigated the molecular crowding effects arising from steric crowding and altered solvent properties on the hammerhead ribozyme activity using water-soluble neutral cosolutes. Poly(ethylene glycol) (PEG) and other… CONTINUE READING

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