Facile synthesis toward the construction of an activity probe library for glycosidases.

@article{Shie2006FacileST,
  title={Facile synthesis toward the construction of an activity probe library for glycosidases.},
  author={Tong-Hong Shie and Ying-Ling Chiang and J Lin and Yaw-Kuen Li and L D Lo},
  journal={Carbohydrate research},
  year={2006},
  volume={341 4},
  pages={443-56}
}
Chemical probes that selectively label the glycoside hydrolase (GH) subfamilies have proven to be a powerful tool in GH-related research. We have previously demonstrated the design and synthesis of an activity probe for beta-glucosidase adopting a cassette-like design in a model study. Herein we report an improved synthetic route using (4-hydroxyphenyl)acetic acid 2-cyanoethyl ester as the precursor for the latent trapping device. Parallel syntheses were performed for the preparation of a… CONTINUE READING