Fabrication of uniform DNA-conjugated hydrogel microparticles via replica molding for facile nucleic acid hybridization assays.

@article{Lewis2010FabricationOU,
  title={Fabrication of uniform DNA-conjugated hydrogel microparticles via replica molding for facile nucleic acid hybridization assays.},
  author={Christina L Lewis and Chang-Hyung Choi and Yan Lin and Chang-Soo Lee and Hyunmin Yi},
  journal={Analytical chemistry},
  year={2010},
  volume={82 13},
  pages={5851-8}
}
We identify and investigate several critical parameters in the fabrication of single-stranded DNA conjugated poly(ethylene glycol) (PEG) microparticles based on replica molding (RM) for highly uniform and robust nucleic acid hybridization assays. The effects of PEG-diacrylate, probe DNA, and photoinitiator concentrations on the overall fluorescence and target DNA penetration depth upon hybridization are examined. Fluorescence and confocal microscopy results illustrate high conjugation capacity… CONTINUE READING
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