Fabrication of transplantable human oral mucosal epithelial cell sheets using temperature-responsive culture inserts without feeder layer cells

@article{BSc2006FabricationOT,
  title={Fabrication of transplantable human oral mucosal epithelial cell sheets using temperature-responsive culture inserts without feeder layer cells},
  author={Daisuke Murakami BSc and Masayuki Yamato and PhD Kohji Nishida and PhD Takeshi Ohki and Ryo Takagi MSc and Joseph Yang BSc and Hideo Namiki and T. Okano},
  journal={Journal of Artificial Organs},
  year={2006},
  volume={9},
  pages={185-191}
}
To exclude bacteria- or animal-derived factors from cultured fabrication of transplantable epithelial cell sheets, primary human oral mucosal epithelial cells were seeded on temperature-responsive culture inserts having submicron-scale pores. Supplying culture medium containing human autologous serum to both apical and basal sides of human epithelial cells allows these cells to grow to confluence. These proliferating cells created stratified epithelial layers even when 3T3 feeder layers and… CONTINUE READING