Fabrication of highly insulating tethered bilayer lipid membrane using yeast cell membrane fractions for measuring ion channel activity.

@article{Jadhav2008FabricationOH,
  title={Fabrication of highly insulating tethered bilayer lipid membrane using yeast cell membrane fractions for measuring ion channel activity.},
  author={Sachin R. Jadhav and D. -D. Sui and R. Michael Garavito and R. Mark Worden},
  journal={Journal of colloid and interface science},
  year={2008},
  volume={322 2},
  pages={465-72}
}
A tethered bilayer lipid membrane (tBLM) was fabricated on a gold electrode using 1,2-dipalmitoyl-sn-glycero-phosphothioethanol as a tethering lipid and the membrane fractions of Saccharomyces pombe yeast cells to deposit the upper leaflet. The membrane fractions were characterized using transmission electron microscopy and dynamic light scattering and found to be similar in size to small unilamellar vesicles of synthetic lipids. The dynamics of membrane-fraction deposition and rupture on the… CONTINUE READING

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