Fabrication of carbohydrate surfaces by using nonderivatised oligosaccharides, and their application to measuring the assembly of sugar-protein complexes.

Abstract

This way up. Dual polarisation interferometry was used to design and characterise a surface on which the orientation and density of immobilised carbohydrates was suitable for studying their interactions with proteins. Lactoferrin was shown to adopt two orientations: "end-on" or "side-on", while for FGF-2 a single monolayer of protein was observed. The new… (More)
DOI: 10.1002/cbic.200800696

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