Fabrication of X-ray compatible microfluidic platforms for protein crystallization.

@article{Guha2012FabricationOX,
  title={Fabrication of X-ray compatible microfluidic platforms for protein crystallization.},
  author={Sudipto Guha and Sarah L Perry and Ashtamurthy S. Pawate and Paul J. A. Kenis},
  journal={Sensors and actuators. B, Chemical},
  year={2012},
  volume={174},
  pages={1-9}
}
This paper reports a method for fabricating multilayer microfluidic protein crystallization platforms using different materials to achieve X-ray transparency and compatibility with crystallization reagents. To validate this approach, three soluble proteins, lysozyme, thaumatin, and ribonuclease A were crystallized on-chip, followed by on-chip diffraction data collection. We also report a chip with an array of wells for screening different conditions that consume a minimal amount of protein… CONTINUE READING