FT-IR study of heterologous protein expression in recombinant Escherichia coli strains.

@article{Ami2003FTIRSO,
  title={FT-IR study of heterologous protein expression in recombinant Escherichia coli strains.},
  author={Diletta Ami and Loredana Bonecchi and Simona Cal{\`i} and Gaetano Orsini and Giancarlo Tonon and Silvia Maria Doglia},
  journal={Biochimica et biophysica acta},
  year={2003},
  volume={1624 1-3},
  pages={6-10}
}
Two recombinant Escherichia coli strains expressing different levels of an interferon fusion protein as inclusion bodies have been studied by Fourier transform infrared (FT-IR) microspectroscopy. A marker band at 1628 cm(-1) allowed monitoring of the protein expression by direct analysis of cell pellets in a rapid, non-invasive and quantitative way. The results demonstrate that FT-IR microspectroscopy is a technique of potential biotechnological interest for studying inclusion body formation. 

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