FRET-based kinetic analysis of highly reactive heterochiral DNA toward EcoRI endonuclease.

@article{Urata2009FRETbasedKA,
  title={FRET-based kinetic analysis of highly reactive heterochiral DNA toward EcoRI endonuclease.},
  author={Hidehito Urata and Chihiro Tamaki and Miki Matsuno and Shun-ichi Wada and Masao Akagi},
  journal={Biochemical and biophysical research communications},
  year={2009},
  volume={390 2},
  pages={192-5}
}
We found unusual reactivity of a heterochiral dodecadeoxynucleotide toward EcoRI that contains an unnatural l-nucleotide residue at the 3'-flanking site of the hexameric EcoRI recognition sequence. In this study, the kinetic parameters for the reaction were determined by means of a heterochiral molecular beacon that contains the EcoRI recognition sequence in the stem region and possesses the fluorescent and quenching dyes at the 5'- and 3'-termini, respectively. We found that the heterochiral… CONTINUE READING