FRET-based imaging of Rac and Cdc42 activation during Fc-receptor-mediated phagocytosis in macrophages.


Fluorescence resonance energy transfer (FRET) imaging can measure the spatial and temporal distributions of activated Rho GTPases within living cells. This information is essential for understanding how signaling networks influence Rho-GTPase switching and for elucidating the mechanisms of Rho GTPase control of the cytoskeleton. This chapter describes FRET… (More)
DOI: 10.1007/978-1-61779-442-1_16


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