FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know?

@article{Mller2010FRAPAK,
  title={FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know?},
  author={Florian M{\"u}ller and Davide Mazza and Timothy J. Stasevich and James G. McNally},
  journal={Current opinion in cell biology},
  year={2010},
  volume={22 3},
  pages={403-11}
}
The binding of nuclear proteins to chromatin in live cells has been analyzed by kinetic modeling procedures applied to experimental data from fluorescence recovery after photobleaching (FRAP). The kinetic models have yielded a number of important biological predictions about transcription, but concerns have arisen about the accuracy of these predictions. First, different studies using different kinetic models have arrived at very different predictions for the same or similar proteins. Second… CONTINUE READING