FRACTIONNEMENT DE L'ORGANE ELECTRIQUE DE LA TORPILLE: LOCALISATION SUBCELLULAIRE DE L'ACETYLCHOLINE SUBCELLULAR FRACTIONATION OF THE ELECTRIC ORGAN OF TORPEDO MARMORATA

@article{Isral1970FRACTIONNEMENTDL,
  title={FRACTIONNEMENT DE L'ORGANE ELECTRIQUE DE LA TORPILLE: LOCALISATION SUBCELLULAIRE DE L'ACETYLCHOLINE SUBCELLULAR FRACTIONATION OF THE ELECTRIC ORGAN OF TORPEDO MARMORATA},
  author={Maurice Isra{\"e}l and Jean Gautron and Bernard Lesbats},
  journal={Journal of Neurochemistry},
  year={1970},
  volume={17}
}
Résumé— L'acètylcholine a été localisée au niveau d'une fraction de vésicules synaptiques isolée à partir de l'organe électrique; le taux d'acétylcholine est fonction de la pression osmotique du milieu. La choline‐acétyltransfèrase (EC 2.3.1.6) n'est pas associée à cette fraction. Les distributions sont comparées à celles de diverses enzymes. 
LES COMPARTIMENTS D'ACETYLCHOLINE DE L'ORGANE ELECTRIQUE DE LA TORPILLE ET LEURS MODIFICATIONS PAR LA STIMULATION
Résumé— L'acétylcholine ‘libre’ et ‘liée’ a été mesurée avant et après stimulation de l'organe électrique de la Torpille in vivo ou in vitro, sur des tranches de tissu incubées dans une solution
[Acetylcholine compartments in stimulated electric organ of Torpedo marmorata].
TLDR
La chute d’acktylcholine ‘libre’ est, comme l’amplitude de the dkharge, dependante du rapport CaZ+/MgZ+.
EVOLUTION DE LA DECHARGE DE L'ORGANE ELECTRIQUE DE LA TORPILLE ET VARIATIONS SIMULTANEES DE L'ACETYLCHOLINE AU COURS DE LA STIMULATION 1 CHANGES IN ACETYLCHOLINE LEVEL AND ELECTROPHYSIOLOGICAL RESPONSE DURING CONTINUOUS STIMULATION OF THE ELECTRIC ORGAN OF TORPEDO MARMORATA
—Au cours d'une période de stimulation, la réponse électrophysiologique de l'organe électrique évolue en trois phases successives: décroissance, palier, décroissance. Le taux d'acétylcholine (ACh)
METABOLISME DU CALCIUM ET LIBERATION DE L'ACETYLCHOLINE DANS L'ORGANE ELECTRIQUE DE LA TORPILLE
TLDR
L'entrée présynaptique de calcium s'accompagne d'une diminution de l'ACh ‘liée’ (vésiculaire) lorsque le tissu est incubé dans une solution exempte de calcium, surtout en présence d'EDTA, the réponse électrophysiologique disparaît.
Loss of vesicular acetylcholine in the Torpedo electric organ on discharge against high external resistance
TLDR
The results confirmed the previous in uiw and irt ilitro observations, and showed in addition that the level of ACh undergoes periodic changes during activity, which appears to be essential for the maintenance of vesicular ACh during repetitive activity.
The electromotor system of Torpedo
TLDR
Studies of the protein composition of the vesicle membrane, especially when combined with similar information about the external presynaptic membrane, promise to throw new light on the molecular mechanism underlying vesicles exo-/endocytosis.
ASPECTS OF ACETYLCHOLINE METABOLISM IN THE ELECTRIC ORGAN OF TORPEDO MARMORATA
TLDR
There was a fraction of acetylcholine in the incubated tissue of higher specific radioactivity that was lost when the tissue was homogenized when electric organ wasHomogenized in iso‐osmotic NaCl‐sucrose unless very potent cholinesterase inhibitors were present.
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References

SHOWING 1-10 OF 28 REFERENCES
The subcellular fractionation of the electric organ of Torpedo
TLDR
The acetylcholine-rich electric organ of Torpedo has been submitted to subcellular fractionation in an attempt to isolate nerve endings and synaptic vesicles derived from cholinergic neurones, but the tissue proved difficult to homogenize and few detached nerve endings were formed.
Synthesis of acetylcholine in the electric organ of Torpedo.
Acetylcholine in the Electric Organ of Torpedo
TLDR
Confirmation of the nature of the active substance and a study of its metabolism in the electric organ are of interest and a reinvestigation of the content of ACh by three different extraction procedures is reinvestigated.
THE FINE STRUCTURE OF THE ELECTRIC ORGAN OF TORPEDO MARMORATA
The fine structure of the electric organ of the fish Torpedo marmorata has been examined after osmium tetroxide or potassium permanganate fixation, acetone dehydration, and Araldite embedment. This
The separation of synaptic vesicles from nerve-ending particles ('synaptosomes').
When brain tissue is homogenized in media iso-osmotic to plasma, the club-like presynaptic nerve endings resist disruption and are snapped or torn off from their attachments to form discrete
THE MORPHOLOGY AND ACETYLCHOLINE CONTENT OF ISOLATED CEREBRAL CORTICAL SYNAPTIC VESICLES
TLDR
In a study of the properties of synaptosomes isolated from various areas of the central nervous system, regional differences in stability were observed during exposure to hypotonic conditions and Synaptosome from the cerebral cortex were found to be more readily disrupted than those from some other areas and to give a higher yield of synaptic vesicles.
The cholinergic nature of the nerves to the electric organ of the Torpedo (Torpedo marmorata)
TLDR
The experiments described in this paper were undertaken in order to discover whether the nerves to the electric organ of the Torpedo act in a similar way to the release of acetylcholine.
Exchangeability of radioactive acetylcholine with the bound acetylcholine of synaptosomes and synaptic vesicles.
TLDR
The exchangeability with added radioactive acetylcholine of the acetylCholine in isolated presynaptic nerve terminals (synaptosomes) and isolated synaptic vesicles was studied by a Sephadex-column method and the possible storage mechanism of vesicular acetyl choline was discussed.
The Electric Organ of the Skate
TLDR
Although the structure and development of the electric organ of the Torpedo has been determined, it is still "impossible to conceive by what steps these wondrous organs have been produced".
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