FM Dye Photo-Oxidation as a Tool for Monitoring Membrane Recycling in Inner Hair Cells

@inproceedings{Kamin2014FMDP,
  title={FM Dye Photo-Oxidation as a Tool for Monitoring Membrane Recycling in Inner Hair Cells},
  author={Dirk Kamin and Natalia H Revelo and Silvio O Rizzoli},
  booktitle={PloS one},
  year={2014}
}
Styryl (FM) dyes have been used for more than two decades to investigate exo- and endocytosis in conventional synapses. However, they are difficult to use in the inner hair cells of the auditory pathway (IHCs), as FM dyes appear to penetrate through mechanotransducer channels into the cytosol of IHCs, masking endocytotic uptake. To solve this problem we applied to IHCs the FM dye photo-oxidation technique, which renders the dyes into electron microscopy markers. Photo-oxidation allowed the… CONTINUE READING

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