FLPe functions in zebrafish embryos

  title={FLPe functions in zebrafish embryos},
  author={Andrew Thomas Wong and Bruce W Draper and Alison L Van Eenennaam},
  booktitle={Transgenic Research},
To assay the efficiency of the FLP/FRT site-specific recombination system in Danio rerio, a construct consisting of a muscle-specific promoter driving EGFP flanked by FRT sites was developed. FLPe capped RNA was microinjected into transgenic single cell stage zebrafish embryos obtained by crossing hemizygous transgenic males with wild-type females. By 48 h post fertilization (hpf), the proportion of embryos displaying green fluorescence following FLPe RNA microinjection was significantly lower… CONTINUE READING