FLP-mediated site-specific recombination for genome modification in turfgrass

@article{Hu2006FLPmediatedSR,
  title={FLP-mediated site-specific recombination for genome modification in turfgrass},
  author={Qian Hu and Kimberly T. Nelson and Hong Wei Luo},
  journal={Biotechnology Letters},
  year={2006},
  volume={28},
  pages={1793-1804}
}
To develop molecular strategies for gene containment in genetically modified (GM) turfgrass, we have studied the feasibility of using the FLP/FRT site-specific DNA recombination system from yeast for controlled genome modification in turfgrass. Suspension cell cultures of creeping bentgrass (Agrostis stolonifera L.) and Kentucky bluegrass (Poa pratensis) were co-transformed with a FLP recombinase expression vector and a recombination-reporter test plasmid containing β-glucuronidase (gusA) gene… CONTINUE READING
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