FLASH Assembly of TALENs Enables High-Throughput Genome Editing

  title={FLASH Assembly of TALENs Enables High-Throughput Genome Editing},
  author={Deepak Reyon and Shengdar Q Tsai and Cyd Khayter and Jennifer A. Foden and Jeffry D. Sander and J. Keith Joung},
  booktitle={Nature Biotechnology},
Engineered transcription activator–like effector nucleases (TALENs) have shown promise as facile and broadly applicable genome editing tools. However, no publicly available high-throughput method for constructing TALENs has been published, and large-scale assessments of the success rate and targeting range of the technology remain lacking. Here we describe the fast ligation-based automatable solid-phase high-throughput (FLASH) system, a rapid and cost-effective method for large-scale assembly… CONTINUE READING
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