FITC-dextran as a probe for endosome function and localization in kidney.

  title={FITC-dextran as a probe for endosome function and localization in kidney.},
  author={Wayne I Lencer and Prof. Dr. J. Weyer and Alan S. Verkman and Dennis A. Ausiello and Dennis L. Brown},
  journal={The American journal of physiology},
  volume={258 2 Pt 1},
Fluorescein isothiocyanate (FITC)-labeled endosomes were localized in kidney epithelial cells after tissue fixation and sectioning, and specific membrane transport properties of isolated endocytic vesicles were measured using the same probe. Rats were infused intravenously with 10 kDa FITC-dextran, and kidneys were fixed with paraformaldehyde lysine periodate. FITC-labeled vesicles were visualized in semithin (1 micron) frozen sections of excised tissue by epifluorescent microscopy and by… CONTINUE READING


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