• Corpus ID: 212473161

FINGERPRINT ANALYSIS FOR VALIDATION AND SIMULTANEOUS QUANTIFICATION OF QUERCETIN AND KAEMPFEROL IN MORINGA OLEIFERA BY RP-HPLC AND HPTLC

@inproceedings{Meghani2018FINGERPRINTAF,
  title={FINGERPRINT ANALYSIS FOR VALIDATION AND SIMULTANEOUS QUANTIFICATION OF QUERCETIN AND KAEMPFEROL IN MORINGA OLEIFERA BY RP-HPLC AND HPTLC},
  author={Nilesh M. Meghani and B D Jethra and R. Shah and Shyamala Nagendran and Shobhaben Pratapbhai Patel},
  year={2018}
}

• N. Meghani, B. Jethra, +2 authors Shobhaben Pratapbhai Patel
• Published 2018

To establish authenticity and integrity for usage of herbal drugs quantitative analysis by sensitive analytical instrument is highly desirable. Quercetin and Kaempferol from whole plant of Moringa oleifera was chosen for simultaneous quantification by RP-HPLC and HPTLC method due to their pharmacological activities. HPTLC method of quercetin and kaempferol was developed on HPTLC plates using Toluene: Ethyl acetate: Chloroform: Formic acid (6:2:5:1.5) as mobile phase. Densitometric evaluation of flavonoids was performed at λ = 240 nm in absorbance mode. Furthermore, RP-HPLC method was developed using mobile phase containing acetonitrile and 0.1 M phosphate buffer (pH 2.5 adjusted by orthophosphoric acid) using C18 column in gradient mode. Chromatographic methods were validated as per ICH guidelines. Excellent linear relationships over the concentration range (1 40 μg/ml) were observed with the r 2 value higher than 0.999 for both the phyto-constituents by RP-HPLC. Limit of detection and quantification were found to be in range of 0.2 0.5 μg/ml with percent recovery of 98 101%; while r 2 value of quercetin and kaempferol were found to be 0.9764 and 0.9823 by HPTLC. The average recovery values for fruits of Moringa oleifera were within range of 98 101% by HPTLC. In conclusion, RP-HPLC method was found to be more precise and sensitive as compared to HPTLC for obtaining acceptable resolution between quercetin and kaempferol. Therefore, HPLC method can be used for the routine analysis of the quality of herbal extracts and formulations of Moringa oleifera. INTRODUCTION: Phytochemical analysis is one of the tools to determine quality which includes preliminary phytochemical screening, chemo profiling and marker compound analysis using advanced analytical techniques. QUICK RESPONSE CODE DOI: 10.13040/IJPSR.0975-8232.9(4).1499-10 Article can be accessed online on: www.ijpsr.com DOI link: http://dx.doi.org/10.13040/IJPSR.0975-8232.9(4).1499-10 HPLC and HPTLC have been established as proficient tools for the phytochemical analysis and shown advanced sensitivity for analytical flexibility for phytoconstituents. Such chromatographic analysis plays an important role in the pharmaceutical development, therefore, there is always a need to develop and validate the analysis method intended to be used for routine analysis 1, 2 . Routinely, plant extracts have been evaluated for their diverse and promising pharmacological properties, and therefore, development of methods for phytochemical analysis