FGF Signaling Inhibition in ESCs Drives Rapid Genome-wide Demethylation to the Epigenetic Ground State of Pluripotency

  title={FGF Signaling Inhibition in ESCs Drives Rapid Genome-wide Demethylation to the Epigenetic Ground State of Pluripotency},
  author={Gabriella Ficz and T. Hore and F. Santos and H. J. Lee and W. Dean and J. Arand and F. Krueger and D. Oxley and Yu-Lee Paul and J. Walter and S. Cook and S. Andrews and M. R. Branco and W. Reik},
  journal={Cell Stem Cell},
  pages={351 - 359}
  • Gabriella Ficz, T. Hore, +11 authors W. Reik
  • Published 2013
  • Biology, Medicine
  • Cell Stem Cell
  • Summary Genome-wide erasure of DNA methylation takes place in primordial germ cells (PGCs) and early embryos and is linked with pluripotency. Inhibition of Erk1/2 and Gsk3β signaling in mouse embryonic stem cells (ESCs) by small-molecule inhibitors (called 2i) has recently been shown to induce hypomethylation. We show by whole-genome bisulphite sequencing that 2i induces rapid and genome-wide demethylation on a scale and pattern similar to that in migratory PGCs and early embryos. Major… CONTINUE READING
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