FG/FxFG as well as GLFG repeats form a selective permeability barrier with self-healing properties

@article{Frey2009FGFxFGAW,
  title={FG/FxFG as well as GLFG repeats form a selective permeability barrier with self-healing properties},
  author={Steffen Frey and Dirk G{\"o}rlich},
  journal={The EMBO Journal},
  year={2009},
  volume={28},
  pages={2554 - 2567}
}
The permeability barrier of nuclear pore complexes (NPCs) controls all nucleo-cytoplasmic exchange. It is freely permeable for small molecules. Objects larger than approximately 30 kDa can efficiently cross this barrier only when bound to nuclear transport receptors (NTRs) that confer translocation-promoting properties. We had shown earlier that the permeability barrier can be reconstituted in the form of a saturated FG/FxFG repeat hydrogel. We now show that GLFG repeats, the other major FG… CONTINUE READING

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Coding sequences and amino acid sequences of proteins used in this study Plasmid name

  • Frey, Görlich
  • 2009
Highly Influential
8 Excerpts

Supplementary Figure S3: Pre-Incubation with an anti-FG repeat antibody drastically reduces the selectivity of an FG/FxFG repeat hydrogel

  • Frey, Görlich
  • 2009
Highly Influential
8 Excerpts

1997b) His6/His10/His14, histidine tag; IBB, importin b-binding domain (corresponding to amino acids 2-63 of Saccharomyces cerevisiae Srp1p); TEV, TEV protease recognition site

  • Kutay
  • 1997
Highly Influential
10 Excerpts

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