FATE OF THYMOCYTES: STUDIES WITH 125I‐IODODEOXYURIDINE AND 3H‐THYMIDINE IN MICE

@article{Joel1977FATEOT,
  title={FATE OF THYMOCYTES: STUDIES WITH 125I‐IODODEOXYURIDINE AND 3H‐THYMIDINE IN MICE},
  author={D. Joel and A. Chanana and H. Cottier and E. Cronkite and J. Laissue},
  journal={Cell Proliferation},
  year={1977},
  volume={10}
}
Cortical thymocytes of young adult mice were labeled in situ with radioactive DNA precursors. As a result of cell emigration and cell death, total thymic radioactivity decreased within 8 days to 10% or less of that present on day 1. Accumulation of thymic migrants in peripheral lymphoid organs was estimated by computing the net thymus‐derived radioactivity in these tissues. Thymic cell death was assessed by comparing values obtained with 125I‐UdR to those acquired with 3H‐TdR. The results… Expand
The Short-Lived Lymphocyte in the Thymus Cortex of the Rat
TLDR
Observations indicate that tritiated deoxycyti-dine labeled cortical thymocytes are short lived and practically none of them recirculate from blood through TDL. Expand
COMPARISON OF DNA RENEWAL IN GERM‐FREE AND CONVENTIONAL MICE USING [125I]IODODEOXYURIDINE AND [3H]THYMIDINE
TLDR
Preursor incorporation was lower in the thymus, mesenteric lymph nodes and femora of GF mice as compared to CV animals and the opposite was observed in the small intestine and skin, i.e. total organ activity was higher in GF animals. Expand
Comparison of cell proliferation in the regenerating bone marrow and spleen of irradiated mice with the use of 125I-iododeoxyuridine.
TLDR
Experiments with measuring the retention of 125IUdR in hemopoietic organs revealed, in the spleen of irradiated animals, phases of increased cell losses as well as immigration of labelled cells into this organ. Expand
Kinetics of mature T-cell development in the thymus.
TLDR
The balance between cell birth, cell maturation, and cell death in the thymus is reexamined by labeling dividing thymocytes and their progeny in vivo with [3H], isolating clearly defined subpopulations by fluorescence-activated cell sorting, and determining the distribution of label by autoradiography. Expand
The Measurement of Cell Turnover Rates Using Stable Isotope‐Labelled Thymidine: Experiments On the Small Intestine and On A Transplantable Lymphatic Tumour of the Mouse
TLDR
The results indicate the feasibility of using thymidine multilabelled with stable isotopes for measurement of cell turnover rates in conjunction with cancer therapy. Expand
Cell proliferation in the bone marrow, thymus and spleen of mice studied by continuous, in vivo bromodeoxycytidine labelling and flow cytometric analysis
TLDR
The results suggest that a larger proportion of spleen cells are long lived than indicated by other methods, and it is found that the period of labelling with BrdUrd extended several days beyond theperiod of infusion. Expand
Haematopoietic antigen‐presenting cells in the human thymic cortex: evidence for a role in selection and removal of apoptotic thymocytes
TLDR
DC‐SIGN+ hAPCs in the human thymic cortex appear to function in thymocyte selection and removal of apoptotic thymocytes from the thymi cortex. Expand
Characterisation of mouse prothymocytes
TLDR
Results obtained in thymus repopulation after transplantation of bone marrow from normal mke did not allow a distinction between the T-cell progenitor function of prothymocytes and that of the pluripotent stem cell determined as CFU-5. Expand
Deficiency of UV-induced excision repair in human thymocytes.
TLDR
The results indicate that immature T-cells are deficient in nucleotide excision repair whereas the majority of mature peripheral blood lymphocytes exhibit such repair. Expand
Mature and Immature Thymocytes
The lineage-specific markers Ly 2 and L3T4, and the homing receptor antigen MEL-14, are used to separate thymocyte subpopulations differing in maturation status. Ly 2–, L3T4– thymocytes proliferateExpand
...
1
2
3
4
...

References

SHOWING 1-10 OF 33 REFERENCES
The fate of thymic radioactivity after local labeling with 125iododeoxyuridine.
TLDR
The data indicated that the vast majority of newly formed thymocytes dies after a short life and only a small fraction of thymic migrants is longer lived. Expand
PROLIFERATIVE ACTIVITY OF THE LYMPHATIC TISSUES OF RATS AS STUDIED WITH TRITIUM-LABELED THYMIDINE
TLDR
Cytokinetic data are presented, employing quantitation of H3DNA in the lymphatic tissues of normal rats serially sacrificed after H3Tdr administration, and there appear to be at least two different types of lymphocytes. Expand
Thymic lymphopoiesis and cell loss in newborn mice.
TLDR
The present data provide support in favor of an extensive emigration of lymphoid cells being the major cause of this cell loss in 2 day old Swiss albino mice. Expand
MAGNITUDE AND PATTERN OF THYMIC LYMPHOCYTE MIGRATION IN NEONATAL MICE
TLDR
It is concluded that a fraction of thymus-derived cells, upon settling in the periphery, remained in, or reentered, a nonproliferative phase for at least 7 days, and the possibility exists that θ-antigen on thymUS-derived lymphocytes may, at least in a fraction, no longer be detectable as they reach the peripheral organs. Expand
Fate of Intraintestinal Thymocytes Labeled with 125lododeoxyuridine or Tritiated Thymidine 1 2
TLDR
In mice injected with cells labeled with 3 HTdR, the initial rapid loss of radioactivity was of shorter duration, and slower during the second phase, presumably due to more extensive reutilization and/or prolonged persistence of acid-soluble radioactivity. Expand
AUTORADIOGRAPHIC ANALYSIS OF LYMPHOPOIESIS AND LYMPHOCYTE MIGRATION IN MICE BEARING MULTIPLE THYMUS GRAFTS
TLDR
It is concluded that the majority of small lymphocytes produced in the thymus andThymus graft tissue do not migrate from these tissues but die locally at the end of their intrathymic life span of 3 to 4 days. Expand
STUDY OF CELL DEATH IN FRIEND LEUKAEMIA
TLDR
The results confirm that most of the cells produced in the Friend leukaemic spleen are rapidly lost; they also demonstrate that this cell loss is mainly due to a massive death, which occurs in proerythro Blastic and erythroblastic compartments after one or two cell divisions. Expand
Fate of H2-activated T lymphocytes in syngeneic hosts. I. Fate in lymphoid tissues and intestines traced with 3H-thymidine, 125I-deoxyuridine and 51chromium.
  • J. Sprent
  • Biology, Medicine
  • Cellular immunology
  • 1976
TLDR
Homing to the intestines seemed a general property of T cells activated to transplantation antigens, which was observed irrespective of whether the T.TDL were activated against H2 determinants, M-locus determinants or H2-plus M- locus determinant. Expand
THYMUS CELL MIGRATION
  • I. Weissman
  • Biology, Medicine
  • The Journal of experimental medicine
  • 1967
TLDR
In the newborn, the kinetic pattern is similar to that of adults, with the single distinction that large cells also migrate, accelerating the tempo of migration in these hosts, and the long-term fate and function of thymus cell migrants has not yet been determined. Expand
Physiological Thymidine Reutilization in Rat Bone Marrow.∗
  • L. Feinendegen, V. Bond, W. Hughes
  • Chemistry, Medicine
  • Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1966
TLDR
Thymidine is confirmed to be a major contributor to the reutilization pathway and the parallel regression of C14-and of H3-labeled DNA after single injection of respectively labeled precursor indicates that kinetic isotope effects do not interfere with the measurement of the re utilization pathway. Expand
...
1
2
3
4
...