FANCI-FANCD2 stabilizes the RAD51-DNA complex by binding RAD51 and protects the 5′-DNA end

  title={FANCI-FANCD2 stabilizes the RAD51-DNA complex by binding RAD51 and protects the 5′-DNA end},
  author={Koichi Sato and Mayo Shimomuki and Yoko Katsuki and Daisuke Takahashi and Wataru Kobayashi and Masamichi Ishiai and Hiroyuki Miyoshi and Minoru Takata and Hitoshi Kurumizaka},
  journal={Nucleic Acids Research},
  pages={10758 - 10771}
The FANCI-FANCD2 (I-D) complex is considered to work with RAD51 to protect the damaged DNA in the stalled replication fork. However, the means by which this DNA protection is accomplished have remained elusive. In the present study, we found that the I-D complex directly binds to RAD51, and stabilizes the RAD51-DNA filament. Unexpectedly, the DNA binding activity of FANCI, but not FANCD2, is explicitly required for the I-D complex-mediated RAD51-DNA filament stabilization. The RAD51 filament… 

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