EyeCi: Optical clearing and imaging of immunolabeled mouse eyes using light‐sheet fluorescence microscopy

@article{Henning2019EyeCiOC,
  title={EyeCi: Optical clearing and imaging of immunolabeled mouse eyes using light‐sheet fluorescence microscopy},
  author={Y. Henning and Christin Osadnik and E. P. Malkemper},
  journal={Experimental Eye Research},
  year={2019},
  volume={180},
  pages={137–145}
}
ABSTRACT Immunofluorescent imaging is an indispensable technique to study morphology and molecular aspects in tissues. Classical approaches make it necessary to cut physical sections of tissue samples to overcome the limited penetration depth of light, restricting the available information to two dimensions. Recent advances in tissue‐clearing techniques enable imaging of fluorescently labeled organs and entire organisms on a cellular level in three dimensions without the need of sectioning… Expand
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