Extremely high titre polyclonal antisera against small neurotransmitter molecules: rapid production, characterisation and use in lightand electron-microscopic immunocytochemistry

@article{Pow1993ExtremelyHT,
  title={Extremely high titre polyclonal antisera against small neurotransmitter molecules: rapid production, characterisation and use in lightand electron-microscopic immunocytochemistry},
  author={David V. Pow and Denise K. Crook},
  journal={Journal of Neuroscience Methods},
  year={1993},
  volume={48},
  pages={51-63}
}
We have produced polyclonal antibodies against the small amino acid neurotransmitters, GABA, glutamate, glycine and taurine, with a simple new technique using antigens co-adsorbed with an adjuvant peptide to gold particles, which causes rapid and massive immune responses in all animals that we have studied. These antibodies are all of extremely high titre; they are typically used in immunocytochemistry at dilutions from 1 in 250,000 to 1 in 1,000,000 which represents an increase in titre of at… CONTINUE READING
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