Extremely Radiation-Resistant Mutants of a Halophilic Archaeon with Increased Single-Stranded DNA-Binding Protein (RPA) Gene Expression

@inproceedings{DeVeaux2007ExtremelyRM,
  title={Extremely Radiation-Resistant Mutants of a Halophilic Archaeon with Increased Single-Stranded DNA-Binding Protein (RPA) Gene Expression},
  author={Linda C DeVeaux and Jochen A. M{\"u}ller and Jonathon E. Smith and Jill E. Petrisko and D Wells and Shiladitya DasSarma},
  booktitle={Radiation research},
  year={2007}
}
Abstract DeVeaux, L. C., Müller, J. A., Smith, J. R., Petrisko, J., Wells, D. P. and DasSarma, S. Extremely Radiation-Resistant Mutants of a Halophilic Archaeon with Increased Single-Stranded DNA-Binding Protein (RPA) Gene Expression. Radiat. Res. 168, 507–514 (2007). Extremely halophilic archaea are highly resistant to multiple stressors, including radiation, desiccation and salinity. To study the basis of stress resistance and determine the maximum tolerance to ionizing radiation, we exposed… 
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85 Citations

Bioengineering radioresistance by overproduction of RPA, a mammalian-type single-stranded DNA-binding protein, in a halophilic archaeon
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Results clearly establish that overexpression of haloarchaeal RPA promotes ionizing radiation resistance in Halobacterium sp.
Divergent Roles of RPA Homologs of the Model Archaeon Halobacterium salinarum in Survival of DNA Damage
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The haloarchaea are unusual in possessing genes for multiple homologs to the ubiquitous single-stranded DNA binding protein (SSB or replication protein A, RPA) found in all three domains of life, and deletions in the five RPA operons are created, suggesting that the different RPA homologys have specialized roles depending on the type of genomic insult encountered.
Nucleotide fluctuation of radiation-resistant Halobacterium sp. NRC-1 single-stranded DNA-binding protein (RPA) genes
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Acid Experimental Evolution of the Haloarchaeon Halobacterium sp. NRC-1 Selects Mutations Affecting Arginine Transport and Catabolism
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In the haloarchaeon NRC-1, as in bacteria, pH adaptation was associated with genes involved in arginine catabolism and proton transport, and this study is among the first to report experimental evolution with multiple resequenced genomes of an archaeon.
Transcriptional profiling of the model Archaeon Halobacterium sp. NRC-1: responses to changes in salinity and temperature
BackgroundThe model halophile Halobacterium sp. NRC-1 was among the first Archaea to be completely sequenced and many post-genomic tools, including whole genome DNA microarrays are now being applied…
Repair of DNA double-strand breaks induced by ionizing radiation damage correlates with upregulation of homologous recombination genes in Sulfolobus solfataricus.
Mechanisms of Resistance to Ionizing Radiation in Extremophiles
TLDR
The role of mannosylglycerate, di-myo-inositol phosphate, and trehalose in the IR resistance of various thermophiles are examined and it is concluded the anaerobic environment confers their IR resistance.
DNA Replication and Repair in Halophiles
The natural environment inhabited by halophiles results in continual exposure of these organisms to elevated temperatures, fluctuations in oxygen and nutrient concentrations, high levels of solar…
Polyploidy in Archaea and Bacteria: About Desiccation Resistance, Giant Cell Size, Long-Term Survival, Enforcement by a Eukaryotic Host and Additional Aspects
  • J. Soppa
  • Biology
    Journal of Molecular Microbiology and Biotechnology
  • 2014
TLDR
The topics include possible laboratory artifacts, resistance against double-strand breaks, long-term survival, relaxation of DNA segregation and septum formation, enforced polyploidy by a eukaryotic host, genome equalization by gene conversion, and the nongenetic usage of genomic DNA as a phosphate storage polymer.
The Survival and Resistance of Halobacterium salinarum NRC-1, Halococcus hamelinensis, and Halococcus morrhuae to Simulated Outer Space Solar Radiation.
TLDR
Overall, this study shows that some halophilic archaea are highly resistant to simulated solar radiation and that they are of high astrobiological significance.
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