Extreme dehydration observed in Antarctic Turgidosculum complicatulum and in Prasiola crispa

  title={Extreme dehydration observed in Antarctic Turgidosculum complicatulum and in Prasiola crispa},
  author={Magdalena Bacior and Piotr Grzegorz Nowak and Hubert Haranczyk and S. Patryas and P. J. Kijak and Agnieszka Ligezowska and Maria A. Olech},
Gaseous phase hydration effect of extremely dehydrated thallus of the Antarctic lichenized fungus Turgidosculum complicatulum and of green alga Prasiola crispa was observed using hydration kinetics, sorption isotherm, 1H-NMR spectroscopy and relaxometry. Three bound water fractions were distinguished: (1) very tightly bound water, (2) tightly bound water and (3) a loosely bound water fraction detected at higher levels of hydration. Sorption isotherm was sigmoidal in form and well fitted using… Expand
Low-temperature immobilization of water in Antarctic Turgidosculum complicatulum and in Prasiola crispa. Part I. Turgidosculum complicatulum.
1H NMR measurements recorded in time and in frequency domain suggest the non-cooperative bound water immobilization in T. complicatulum thallus is a result of diffusion induced migration of separated water molecules to ice microcrystallites already present inThallus, but still beyond the calorimeter resolution. Expand
A comparative analysis of gaseous phase hydration properties of two lichenized fungi: Niebla tigrina (Follman) Rundel & Bowler from Atacama Desert and Umbilicaria antarctica Frey & I. M. Lamb from Robert Island, Southern Shetlands Archipelago, maritime Antarctica
Gaseous phase hydration properties for thalli of Niebla tigrina from Atacama Desert, and for Umbilicaria antarctica from Isla Robert, maritime Antarctica, were analyzed using 1H-NMR relaxometry,Expand
Symbiosis at its limits: ecophysiological consequences of lichenization to the genus Prasiola in Antarctica.
The results support an improved tolerance to subzero temperature as main benefit of lichenization for the photobiont, but highlight thatLichenization represents a delicate equilibrium between a mutualistic and a less reciprocal relationship. Expand
Dehydration and Freezing Resistance of Lichenized Fungi
The poikilohydrous nature of lichens provides them the ability to resist low temperature, deep dehydration and deficit in light irradiance. The process of water uptake can be correlated with theExpand
In vivo anti-inflammatory effects of Prasiola japonica ethanol extract
Abstract The aim of this study was to investigate the efficacy of Prasiola japonica ethanol extract (Pj-EE) in various mouse inflammation models. For edema mice models, eight-week-old female ICR miceExpand


A method of water-soluble solid fraction saturation concentration evaluation in dry thalli of Antarctic lichenized fungi, in vivo
Although in dehydrated C.aculeata the level of carbohydrates and polyols was low, the lichenase action during rehydration process increased it; the averaged saturation concentration cs, which resembled that for sucrose, may be applied for other organisms experiencing extreme dehydration or for dry tissues. Expand
Deep dehydration of Umbilicaria aprina thalli observed by proton NMR and sorption isotherm
Abstract The initial stages of Umbilicaria aprina Nyl. hydration (starting from the hydration level Δm/m0 = 0.048 ± 0.004) were observed using hydration kinetics, sorption isotherm and proton NMR.Expand
Initial Phases of Antarctic Ramalina terebrata Hook f. & Taylor Thalli Rehydration Observed by Proton Relaxometry
Hydration kinetics, sorption isotherm, and proton free induction decays are measured for Ramalina terebrata thalli rehydrated from gaseous phase. Very tightly, tightly, and loosely bound waterExpand
Bound Water Structure on the Surfaces of Usnea antarctica as Observed by NMR and Sorption Isotherm
Antarctic lichens are extremophilic organisms very resistant on desiccation stress and cold [1–3]. They perform active photosynthesis below 0◦C [4, 5] and below ice nucleation of their cellularExpand
Deep Dehydration of Antarctic Lichen Leptogium puberulum Hue Observed by NMR and Sorption Isotherm
Hydration kinetics, sorption isotherm, and proton free induction decays are measured for Leptogium puberulum Hue thalli hydrated from gaseous phase. Very tightly, tightly and loosely bound waterExpand
Physiology and Ultrastructure of Desiccation in the Green Alga Prasiola crispa from Antarctica
Physiological and fine structural responses of the antarctic green alga Prasiola crispa ssp. antarctica (Kutz.) Knebel to desiccation and reimmersion in seawater are described. Experiments wereExpand
13C NMR analysis of Antarctic cryptogam extracts
Water soluble compounds were extracted from the dominant cryptogams of the Windmill Islands, Wilkes Land, and compared with standard polyols, sugars and amino acids using 13C nuclear magneticExpand
The Investigation of Hydration Processes in Horse Chestnut (Aesculus hippocastanum L.) and Pine (Pinus silvestris L.) Bark and Bast Using Proton Magnetic Relaxation
Summary The proton free induction decay (FID) was shown to be effective in monitoring of hydration and the water soluble extractive fraction in horse chestnut (Aesculus hippocastanum, L.) and pineExpand
Photosynthesis in a sub-Antarctic shore-zone lichen.
Photosynthetic responses to moisture, light, temperature, salinity and inorganic nitrogen fertilization are reported for a shore-zone lichen Turgidiusculum complicatulum, a possible recent introduction to sub-Antarctic Marion Island. Expand
The accumulation of proline in Prasiola crispa during winter in Antarctica
Measurements of photosynthetic quantum yield indicated that winter samples of P. crispa also recovered photosynthesis activity upon thawing, and it seems probable that proline is involved in cryoprotection in this species. Expand