Extraintestinal Pathogenic and Antimicrobial-Resistant Escherichia coli Contamination of 56 Public Restrooms in the Greater Minneapolis-St. Paul Metropolitan Area

  title={Extraintestinal Pathogenic and Antimicrobial-Resistant Escherichia coli Contamination of 56 Public Restrooms in the Greater Minneapolis-St. Paul Metropolitan Area},
  author={Muhanad Mohamed and Krista Owens and Abby Gajewski and Connie R. Clabots and Brian D. Johnston and Paul D. Thuras and Michael A. Kuskowski and James R. Johnson},
  journal={Applied and Environmental Microbiology},
  pages={4498 - 4506}
ABSTRACT How extraintestinal pathogenic Escherichia coli (ExPEC) and antimicrobial-resistant E. coli disseminate through the population is undefined. We studied public restrooms for contamination with E. coli and ExPEC in relation to source and extensively characterized the E. coli isolates. For this, we cultured 1,120 environmental samples from 56 public restrooms in 33 establishments (obtained from 10 cities in the greater Minneapolis-St. Paul, MN, metropolitan area in 2003) for E. coli and… Expand
Phylogenetic Backgrounds and Virulence-Associated Traits of Escherichia coli Isolates from Surface Waters and Diverse Animals in Minnesota and Wisconsin
The authors' detection of potentially pathogenic strains that may pose a health threat to humans among E. coli isolates from surface waters and wild and domesticated animals suggests a need for heightened attention to these reservoirs as possible sources for human acquisition of disease-causing E. bacteria. Expand
Prevalence and Molecular Characteristics of Avian-Origin mcr-1-Harboring Escherichia coli in Shandong Province, China
E. coli from poultry in Shandong could be a reservoir for the mcr-1 gene, which could pose serious risks to human public health, according to this study. Expand
Accessory Traits and Phylogenetic Background Predict Escherichia coli Extraintestinal Virulence Better Than Does Ecological Source
Among human-source E. coli isolates, specific accessory traits and phylogenetic/clonal backgrounds predict experimental virulence in a murine sepsis model better than does ecological source. Expand
Public Toilets in a tertiary institution in the Southern part of Nigeria as Potential Reservoirs of Drug Resistant Pathogens
This study shows that public toilets could play a role not just as a reservoir of potential pathogens but more specifically as a potential reservoir of drug resistant pathogenic microorganisms with high MAR indices. Expand
Isolation and Characterization of Escherichia coli from Animals, Humans, and Environment
This chapter outlines basics of Escherichia coli isolation and characterization strategies that can assist in research designing that matches the set objectives. Expand
Transmission of COVID-19 and other infectious diseases in public washrooms: A systematic review
Although there is a risk of microbial aerosolisation from toilet flushing and the use of hand drying systems, there is no evidence of airborne transmission of enteric or respiratory pathogens, including COVID-19, in public washrooms. Expand
Assessing Occupational Exposure to Surface Contaminants in Kuwaiti Educational Buildings
The prevalence of surface contaminants, such as potentially harmful bacteria, within building environments in the State of Kuwait is not known.To the authors’ knowledge, this article is the frst ofExpand


Isolation and Molecular Characterization of Nalidixic Acid-Resistant Extraintestinal Pathogenic Escherichia coli from Retail Chicken Products
The similarity of the Nal-resistant and -susceptible populations suggests that they derive from the same source population, presumably the avian fecal flora, with Nal resistance emerging by spontaneous mutation as a result of fluoroquinolone exposure. Expand
Epidemic Clonal Groups of Escherichia coli as a Cause of Antimicrobial-Resistant Urinary Tract Infections in Canada, 2002 to 2004
Findings indicate that among the E. coli urine isolates studied, resistance to TS and FQs has a prominent clonal component, with the O15:K52:H1 clonal group and especially E. bacteria ST131 being the major contributors. Expand
Dissemination of ST131 and ST393 community-onset, ciprofloxacin-resistant Escherichia coli clones causing urinary tract infections in Korea.
Based on genotyping, it is suggested that epidemic and virulent ciprofloxacin-resistant E. coli clones such as ST131 and ST393 have disseminated in Korea. Expand
A BSTRACT Background The management of urinary tract infections is complicated by the increasing prevalence of antibiotic-resistant strains of Escherichia coli. We studied the clonal composition ofExpand
Extraintestinal Pathogenic Escherichia coli: A Combination of Virulence with Antibiotic Resistance
  • J. Pitout
  • Biology, Medicine
  • Front. Microbio.
  • 2012
This review will highlight aspects of virulence associated with ExPEC, provide a brief overview of plasmid-mediated resistance to β-lactams including the characteristics of the successful international sequence types such as ST38, ST131, ST405, and ST648 among ExPec. Expand
Pandemic lineages of extraintestinal pathogenic Escherichia coli.
  • L. Riley
  • Biology, Medicine
  • Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
  • 2014
Major pandemic clonal lineages of ExPEC, associated with both community-onset and healthcare-associated infections, are described based on multilocus sequence typing (MLST), and possible reasons for their global dissemination are discussed. Expand
Multidrug-Resistant Urinary Tract Isolates ofEscherichia coli: Prevalence and Patient Demographics in the United States in 2000
Conurrent resistance to antimicrobials of different structural classes has arisen in a multitude of bacterial species and may complicate the therapeutic management of infections, including those of the urinary tract, and continued local, regional, and national surveillance is warranted. Expand
European Emergence of Ciprofloxacin-Resistant Escherichia coli Clonal Groups O25:H4-ST 131 and O15:K52:H1 Causing Community-Acquired Uncomplicated Cystitis
A total of 148 E. coli strains displaying reduced susceptibility to ciprofloxacin and causing uncomplicated urinary tract infections in eight European countries during 2003 to 2006 were studied, revealing a substantial heterogeneity in the phylogenetic groups, biochemical profiles, and antibiotic susceptibilities. Expand
Escherichia coli sequence type ST131 as the major cause of serious multidrug-resistant E. coli infections in the United States.
BACKGROUND Escherichia coli sequence type ST131 (O25:H4), associated with the CTX-M-15 extended-spectrum beta-lactamase, has emerged internationally as a multidrug-resistant pathogen but has receivedExpand
Intercontinental emergence of Escherichia coli clone O25:H4-ST131 producing CTX-M-15.
A broadly disseminated, CTX-M-15-positive and virulent E. coli clonal group with highly homogeneous virulence genotypes and subgroups exhibiting highly similar PFGE profiles is described, suggesting recent emergence. Expand