Extracts of kava (Piper methysticum) induce acute anxiolytic-like behavioral changes in mice

  title={Extracts of kava (Piper methysticum) induce acute anxiolytic-like behavioral changes in mice},
  author={Kennon M. Garrett and Garo P. Basmadjian and Ikhlas A. Khan and Brian T. Schaneberg and Thomas W. Seale},
RationaleKava has been used for centuries by Pacific Islanders for its tranquilizing and sedative effects. [] Key MethodMethodsVarious doses of an ethanolic extract of kava root or diazepam were administered intraperitoneally to BALB/cByJ inbred mice. Behavioral changes were measured in the mirrored chamber avoidance assay and elevated plus-maze assay. Reduced latency to enter and increased time spent in a normally avoided environment operationally defined anxiolysis.

Neuropharmacological studies of Piper auritum Kunth (Piperaceae): antinociceptive and anxiolytic-like effects

It is demonstrated for the first time that both organic and aqueous extracts of the leaves of P. auritum possess antinociceptive effect while at higher doses the organic extracts exert a depressant effect on the CNS in mice.

Anxiolytic-like effect of the leaves of Pseudospondias microcarpa (A. Rich.) Engl. in mice

Results of the present study indicate that PME possesses anxiolytic-like effects in mice, suggesting sedative and anticonvulsant effects and reducing the stress-induced increase in rectal temperature.

Sedative and anxiolytic effects of the extracts of the leaves of Stachytarpheta cayennensis in mice.

Flumazenil, blocked the effect of the leaves of Stachytarpheta cayennensis on rearing, locomotion and elevated plus maze suggesting that GABA receptors are involved in the observed sedative and anxiolytic activities.

Neurobehavioral and toxicological activities of two potentially CNS-acting medicinal plants of Piper genus.

Evaluation of Anti-Convulsive Properties of Aqueous Kava Extract on Zebrafish Using the PTZ-Induced Seizure Model

Results indicate that aqueous extract of Kava stems without peel after 45 min of pre-treatment exhibited anti-convulsive potential at the dose of 50 mg/L.

Anxiolytic action and safety of Kava: Effect on rat brain acetylcholinesterase activity and some serum biochemical parameters

The cholinergic system in the cortex, hippocampus and striatum may play a vital role in the anxiolytic action of kava, as measured by measuring acetylcholinesterase activity in adult male rats.

Kava and its Kavalactones Inhibit Norepinephrine-induced Intracellular Calcium Influx in Lung Cancer Cells.

Results show that kava extract effectively inhibits NE-mediated intracellular calcium influx in H1299 cells, potentially through antagonizing β-AR signaling, and suggests a novel mechanism through which kava and its ingredients potentially offer the anxiolytic and cancer-preventive activity.

Kavain, the Major Constituent of the Anxiolytic Kava Extract, Potentiates GABAA Receptors: Functional Characteristics and Molecular Mechanism

This study characterised the functional properties of the major anxiolytic kavalactone, kavain at human recombinant α1β2, β2γ2L, αxβ2γ 2L and α4β2δ GABAARs expressed in Xenopus oocytes using the two-electrode voltage clamp technique, and presents the first experimental evidence in support of a direct interaction between a kvalactone and GABA ARs.



Anxiolytic-like effects of Kava-Kava in the elevated plus maze test—a comparison with diazepam

Anxiolytics by ethanol, diazepam and buspirone in a novel murine behavioral assay.

Findings indicate that the murine mirrored chamber assay responds to several agents known to be anxiolytic in man but differs from the plus-maze in the pharmacological spectrum of the anxIOlytics to which it is sensitive.

Effect of kava extract and individual kavapyrones on neurotransmitter levels in the nucleus accumbens of rats

Comparison of the central nervous system activity of the aqueous and lipid extract of kava (Piper methysticum).

The pharmacological effects of kava ingestion appear to be due to the activity of the compounds present in the lipid-soluble fraction, which produces a greater range of pharmacological actions than the aqueous extract, and the latter is orally inactive in mice and rats.

Effect of aqueous and lipid-soluble extracts of kava on the conditioned avoidance response in rats.

The aqueous, pyrone-free extract from kava (Piper methysticum) and the lipid-soluble extract (kava resin) were tested for their effect on amphetamine-induced hypermotility in mice and on conditioned

Extract of kava (Piper methysticum) and its methysticin constituents protect brain tissue against ischemic damage in rodents.

Interaction of various Piper methysticum cultivars with CNS receptors in vitro.

Investigation indicates that the GABAA, dopamine D2, opioid (mu and delta) and histamine (H1 and H2) receptors might be involved in the pharmacological action of kava extracts.

Kavapyrone enriched extract fromPiper methysticum as modulator of the GABA binding site in different regions of rat brain

The findings suggest that one way kavapyrones might mediate sedative effects in vivo is through effects on GABAA receptor binding, i.e., synergetic effect on [3H] muscimol binding.

Kava pyrones and resin: studies on GABAA, GABAB and benzodiazepine binding sites in rodent brain.

The pharmacological activities of kava resin and pyrones do not appear to be explained by any significant interaction with GABA or benzodiazepine binding sites, and this did not correlate with pharmacological activity.

Genotypic Differences Between C57BL/6 and A Inbred Mice in Anxiolytic and Sedative Actions of Diazepam

Findings indicate that C57BL/6J and A/J mice provide a valuable tool for behavioral genetic studies of the mechanisms underlying the pharmacological actions of benzodiazepines.