Extracts from Fermented Black Soybean Milk Exhibit Antioxidant and Cytotoxic Activities


In this study, ethanol extracts from 2-day fermented black soybean milk (FBE) by immobilized Rhizopus oligosporus NTU5 have been evaluated for both antioxidant and cytotoxic activities. The results reveal that a 2-day FBE had strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging effect (76 %). The extracts were further fractionated by silica gel column chromatography and an unknown compound, FBE5-A, was obtained, which exhibited strong antioxidant activity. IC50 of the DPPH scavenging effect of FBE5-A was 7.5 mg/mL, which is stronger than a commonly used antioxidant, vitamin E (a-tocopherol; 17.4 mg/mL), and similar to vitamin C (ascorbic acid; 7.6 mg/mL). The cytotoxic test demonstrated that extracts of 2-day fermented broth exhibited selective cytotoxic activity towards human carcinoma cells, Hep 3B (IC50=150.2 mg/mL), and did not affect normal human lung fibroblasts, MRC-5 (p<0.05). The results indicate the potential applications of fermented black soybean milk as functional food, pharmaceutical or cancer therapy formula.

5 Figures and Tables


Citations per Year

62 Citations

Semantic Scholar estimates that this publication has 62 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@inproceedings{Cheng2011ExtractsFF, title={Extracts from Fermented Black Soybean Milk Exhibit Antioxidant and Cytotoxic Activities}, author={Kuan-Chen Cheng and Jiun-Tsai Lin and Wen-Hsiung Liu}, year={2011} }