Extraction of sleep-promoting factor S from cerebrospinal fluid and from brains of sleep-deprived animals.

@article{Pappenheimer1975ExtractionOS,
  title={Extraction of sleep-promoting factor S from cerebrospinal fluid and from brains of sleep-deprived animals.},
  author={John R. Pappenheimer and Gary Koski and V Fencl and Manfred L. Karnovsky and James M. Krueger},
  journal={Journal of neurophysiology},
  year={1975},
  volume={38 6},
  pages={1299-311}
}
Sleep-promoting factor (factor S) was extracted, partially purified, and concentrated from cerebrospinal fluid and from acid-acetone extracts of brain stem anc cortex of sleep-deprived goats and sheep. 2. Solutes greater than 500 daltons were largely removed by serial ultrafiltrations through molecular sieves (Amicon membranes UM10 and UM05); solutes less than 350 daltons were largely eliminated by gel filtration through Sephadex G10 columns. Sleep-promoting activity was found in a fraction… CONTINUE READING

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