Extraction of proteins from biological fluids by use of an ionic liquid/aqueous two-phase system.

@article{Du2007ExtractionOP,
  title={Extraction of proteins from biological fluids by use of an ionic liquid/aqueous two-phase system.},
  author={Zhuo Du and Yong-Liang Yu and Jian-Hua Wang},
  journal={Chemistry},
  year={2007},
  volume={13 7},
  pages={
          2130-7
        }
}
An ionic liquid/aqueous two-phase system based on the hydrophilic ionic liquid 1-butyl-3-methylimidazolium chloride (BmimCl) and K(2)HPO(4) has been employed for direct extraction of proteins from human body fluids for the first time. Proteins present at low levels were quantitatively extracted into the BmimCl-rich upper phase with a distribution ratio of about 10 between the upper and lower phase and an enrichment factor of 5. Addition of an appropriate amount of K(2)HPO(4) to the separated… CONTINUE READING

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