Extraction of nuclear proteins from root meristematic cells.

  title={Extraction of nuclear proteins from root meristematic cells.},
  author={Fernando Gonz{\'a}lez-Camacho and F Javier Medina},
  journal={Methods in molecular biology},
Fractionation and extraction of nuclear proteins are techniques intended to facilitate dedicated plant proteomic studies. These techniques rely on subcellular fractionation, which makes it possible to define and characterize the proteome of a subcellular organelle, in this case the cell nucleus. Nuclear protein fractionation is proposed as a method to be carried out according to the solubility of proteins in buffers of increasing ionic strength. This physical criterion, accompanied in some… CONTINUE READING
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