Extraction of membrane proteins from a living cell surface using the atomic force microscope and covalent crosslinkers

@article{Afrin2003ExtractionOM,
  title={Extraction of membrane proteins from a living cell surface using the atomic force microscope and covalent crosslinkers},
  author={Rehana Afrin and Hideaki Arakawa and Toshiaki Osada and Atsushi Ikai},
  journal={Cell Biochemistry and Biophysics},
  year={2003},
  volume={39},
  pages={101-117}
}
The force curve mode of the atomic force microscope (AFM) was applied to extract intrinsic membrane proteins from the surface of live cells using AFM tips modified by amino reactive bifunctional covalent crosslinkers. The modified AFM tips were individually brought into brief contact with the living cell surface to form covalent bonds with cell surface molecules. The force curves recorded during the detachment process from the cell surface were often characterized by an extension of a few… CONTINUE READING
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