Extraction of label during processing of brain tissue for electron microscopic autoradiographic investigation of glycoprotein metabolism

@article{Pilgrim1982ExtractionOL,
  title={Extraction of label during processing of brain tissue for electron microscopic autoradiographic investigation of glycoprotein metabolism},
  author={Ch. Pilgrim and I. Saalm{\"u}ller and Ingrid Reisert},
  journal={Histochemistry},
  year={1982},
  volume={74},
  pages={271-278}
}
Rats were injected intracerebrally with (3H) fucose and survived for 30 min and 24 h. Following perfusion fixation with aldehydes, Vibratome sections of the brains were processed for embedding in resin and the radioactivity in the various solutions measured by scintillation counting. The decline of radioactivity was monitored in up to 18 successive buffer… CONTINUE READING