Extraction of high-quality genomic DNA from Ectocarpus.

  title={Extraction of high-quality genomic DNA from Ectocarpus.},
  author={Susana M Coelho and Delphine Scornet and Sylvie Rousvoal and Nick T. Peters and Laurence Dartevelle and Akira F Peters and J. Mark Cock},
  journal={Cold Spring Harbor protocols},
  volume={2012 3},
For some applications, such as genome sequencing and high-throughput genotyping with multiple markers, it is necessary to use high-quality genomic DNA. This article describes how to obtain several micrograms of high-quality, cesium chloride-purified DNA from 1 g of Ectocarpus filaments. We also recommend using DNA of this quality for quantitative RT-PCR control reactions. However, simpler, more rapid, kit-based methods are preferable for experiments that involve the treatment of large numbers… CONTINUE READING