Extraction of exopolymers from biofilms: the protective effect of glutaraldehyde.

  title={Extraction of exopolymers from biofilms: the protective effect of glutaraldehyde.},
  author={Joana C. Azeredo and M. Henriques and Sanna M. Sillankorva and Ros{\'a}rio T. Oliveira},
  journal={Water science and technology : a journal of the International Association on Water Pollution Research},
  volume={47 5},
The extraction of the exopolymeric matrix is a prerequisite to properly assessing the composition of the biofilm. Several extraction methods have already been developed, however, no universal method has yet been adopted because the compromise between high yields of extraction and minimum cell lysis is difficult to establish. In fact, most of the extraction methods promote leakage of intracellular material. The most common extraction methods, Dowex resin and sonication, were assayed in biofilms… CONTINUE READING