Extraction of RNA from tissues containing high levels of procyanidins that bind RNA

@article{Wang1994ExtractionOR,
  title={Extraction of RNA from tissues containing high levels of procyanidins that bind RNA},
  author={Chang-Sheng Wang and Lila O. Vodkin},
  journal={Plant Molecular Biology Reporter},
  year={1994},
  volume={12},
  pages={132-145}
}
Commonly used methods for extraction of RNA from plants are not effective for isolation of high quality RNA from the pigmented seed coats of soybeans that produce procyanidins (tannins) during seed coat development. We demonstrate a significant modification of the phenol-LiCl method that yields high quality RNA from a black seed coat variety. In this method, seed coat material was ground in a buffer containing a high concentration of bovine serum albumin (100 mg BSA/50 mg of lyophilized seed… CONTINUE READING

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