Extraction of RNA from cheese without prior separation of microbial cells.

  title={Extraction of RNA from cheese without prior separation of microbial cells.},
  author={Christophe Monnet and Vincent M Ulv{\'e} and A S Sarthou and Françoise Irlinger},
  journal={Applied and environmental microbiology},
  volume={74 18},
In situ gene expression studies are promising approaches for improving our understanding of the cheese microbial flora. This requires efficient RNA extraction methods, but studies of cheeses are scarce. The objective of the present study was to determine whether RNA samples compatible with quantitative mRNA transcript analyses can be obtained without separating the cells from the cheese matrix. In the method that we describe, the cellular processes are stopped at the very beginning of the… CONTINUE READING
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