Extraction of RAPD-friendly DNA from Laminaria japonica (Phaeophyta) after enzymatic dissociation of the frozen sporophyte tissues

@article{Hu2004ExtractionOR,
  title={Extraction of RAPD-friendly DNA from Laminaria japonica (Phaeophyta) after enzymatic dissociation of the frozen sporophyte tissues},
  author={Y.-J. Hu and Z Zhou},
  journal={Journal of Applied Phycology},
  year={2004},
  volume={13},
  pages={415-422}
}
A new extraction protocol has been developed to obtain high quality DNAfrom Laminaria japonica, which involves enzymatic dissociation ofsporophyte tissues and subsequent elimination of the remainingpolysaccharides with cetyltrimethyl ammonium bromide. Unicells isolatedfrom frozen kelp tissues with alginate lyase prepared from the abalone Haliotis diversicolor were used to extract total DNA; the yield wasapproximately 13 to 22.5 μg DNA g-1 (wet sporophyteweight). The average size of genomic DNA… CONTINUE READING