Extraction of IgY from egg yolk using a novel aqueous two-phase system and comparison with other extraction methods.

@article{Stlberg2001ExtractionOI,
  title={Extraction of IgY from egg yolk using a novel aqueous two-phase system and comparison with other extraction methods.},
  author={J St{\aa}lberg and Anders Larsson},
  journal={Upsala journal of medical sciences},
  year={2001},
  volume={106 2},
  pages={99-110}
}
Egg yolk is an important source of antibodies. The biggest obstacle for isolation of chicken antibodies (IgY) is the removal of lipids, which are present in abundance in egg yolk. We have used a two-phase system to separate egg yolk. The use of an aqueous two-phase system with phosphate and Triton X-100 made separation of lipids and water-soluble proteins possible. Lipids are extracted into the detergent-enriched top-phase, whereas IgY is isolated in the phosphate-enriched bottom-phase. The… CONTINUE READING