[Extraction and purification of Schwann cells cytoplasmic neurotrophic proteins with high pressure liquid chromatography and study on its neurobiological activities].

Abstract

OBJECTIVE To purify and study Schwann cells cytoplasmic neurotrophic protein. METHODS The dissociated SC taken from 300 newborn rats sciatic nerves were cultured, collected, ultrasonicated and ultraspeed centrifuged. The supernates were ultrafiltrated and concentrated by using ultrafiltration units with PM10, PM30, PM50 ultrafiltration membranes. The… (More)

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@article{Xie2000ExtractionAP, title={[Extraction and purification of Schwann cells cytoplasmic neurotrophic proteins with high pressure liquid chromatography and study on its neurobiological activities].}, author={Guoxiong Xie and Ji Zhu and Xue Feng Gu}, journal={Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery}, year={2000}, volume={14 4}, pages={226-9} }