Extraction and preliminary characterization of a human bronchogenic carcinoma antigen.

  title={Extraction and preliminary characterization of a human bronchogenic carcinoma antigen.},
  author={Michael James Frost and G. T. Rogers and Kenneth Dawson Bagshawe},
  journal={British Journal of Cancer},
  pages={379 - 386}
Saline extracts of human bronchogenic tumours, soluble in 50% saturated ammonium sulphate and also fractions from Sephadex G-200 chromatography were used to raise antisera in rabbits. After absorbing the antisera with normal tissue extracts, direct Ouchterlony tests were performed against tumour (adenocarcinomata and squamous cell carcinomata) and normal extracts. A precipitin reaction was given with all 11 tumour extracts tested at a concentration of 5 mg/ml whereas all the 9 normal lung… 

Preparation and characterization of an antiserum to cultured human oat-cell carcinoma cells.

Removal of anti-CEA antibodies by absorption with purified CEA has not reduced the cross-reactivity of the absorbed antiserum with the panel of human lung-cancer cells.

Identificationand Purificationof a HumanLungTumor . associated Antigenfrom a PrimaryLungTumor

A human lung tumor-associated protein has been purified from an extract of a human small cell carcinoma of the lung and shown by Ouchterlony double diffusion analysis to be antigenically identical to

Isolation and Identification of Human Lung Tumor-associated Antigens 1

The isolation, characterization, and identifica tion of 3 human lung TM's found in significant concentra tions in several histopathological types of lung tumors are reported.

A plasma membrane antigen highly associated with oat‐cell carcinoma of the lung and undetectable in normal adult tissue

It is concluded that oat‐cell carcinomas of the lung express a plasma membrane antigen (s) undetectable in normal adult tissue and highly associated with this tumor type.

A human tumour-associated membrane antigen from squamous-cell carcinoma of the lung

Lung TAMA-1 was detected in 80% of lung tumour Triton-X-100 extracts tested by counterimmuno-electrophoresis (CIEP) but was undetectable in breast or colon carcinoma extracts, and has a cathodal gamma-type electrophoretic mobility at pH 7·6.

Isolation and Identification of Human Lung Tumor-associated Antigens1

Lung TAA 1 was partially adsorbed and cross-reacted with certain normal serum or plasma prepara tions used and appears to be a normal serum protein in Cohn Fraction IV-4, and lung TAA 2 and 3 appear only in lung tumor-soluble extracts, whereas the lung T AA 1 was de monstrable in soluble extracts of breast, colon, cervical, and head and neck carcinoma.




It was shown that the tumor-specific antibodies were not directed against bacterial contaminants or against the unusually high concentrations of fibrin found in many neoplastic tissues.

Carcinoembryonic antigenic activity of tissue extracts: A quantitative study of malignant and benign neoplasms, cirrhotic liver, normal adult and fetal organs

The results indicated that CEA was present in varying amounts in a variety of tumors the identification of these reactive substances will require more precise physico‐chemical and antigenic characterization.

The Use of Immunoadsorbent Columns for the Isolation of Antibodies Specific for Antigens Associated with Human Bronchogenic Carcinoma

An immunoadsorbent technique is described whereby tumour-specific antibodies may be isolated and raised in rabbits to a variety of extracts from human bronchogenic carcinoma to yield antisera specific for tumours-associated antigens.

Agarose-gel electrophoresis of soluble proteins from bronchial mucosa and bronchogenic carcinoma.

The soluble cytoplasmic proteins isolated from bronchial mucosae and 15 bronchogenic carcinomas of human origin have been examined by agarose-gel electrophoresis and compared with a similar protein pr

Embryonic Origin of Human Tumor-Specific Antigens

Foetal Aiitigeiis ill Cancer

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The Future of Tumor ImInunology

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