Extraction and partial purification of acyl-CoA:1-acyl-sn-glycero-3-phosphocholine acyltransferase from rat liver microsomes.

@article{HasegawaSasaki1980ExtractionAP,
  title={Extraction and partial purification of acyl-CoA:1-acyl-sn-glycero-3-phosphocholine acyltransferase from rat liver microsomes.},
  author={H Hasegawa-Sasaki and Kousaku Ohno},
  journal={Biochimica et biophysica acta},
  year={1980},
  volume={617 2},
  pages={205-17}
}
Acyl-CoA:1-acyl-sn-glycero-3-phosphocholine acyltransferase (EC 2.3.1.23) was extracted from rat liver microsomes with an aqueous dispersion of 1-acyl-sn-glycero-3-phosphocholine, a substrate of the enzyme, and purified up to 30-fold. The procedure includes removal of unrelevant proteins and lipids by washings of microsomes with a buffer of high ionic strength and with buffers containing detergents, extraction of the enzyme with an aqueous dispersion of 1-acyl-sn-glycero-3-phosphocholine, and… CONTINUE READING