• DOI:10.1016/S0076-6879(07)32006-5
Corpus ID: 21105062

Extraction and analysis of sterols in biological matrices by high performance liquid chromatography electrospray ionization mass spectrometry.

@article{McDonald2007ExtractionAA,
  title={Extraction and analysis of sterols in biological matrices by high performance liquid chromatography electrospray ionization mass spectrometry.},
  author={J. McDonald and Bonne M. Thompson and Erin C McCrum and D. Russell},
  journal={Methods in enzymology},
  year={2007},
  volume={432},
  pages={
          145-70
        }
}

J. McDonald, Bonne M. Thompson, +1 author D. Russell

Published 2007

Chemistry, Medicine

Methods in enzymology

We describe the development of a high performance liquid chromatography mass spectrometry (HPLC-MS) method that allows the identification and quantitation of sterols in mammalian cells and tissues. Bulk lipids are extracted from biological samples by a modified Bligh/Dyer procedure in the presence of eight deuterated sterol standards to allow subsequent quantitation and determination of extraction efficiency. Sterols and other lipids are resolved by HPLC on a reverse-phase C18 column using a… CONTINUE READING

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