Extraction and analysis of sterols in biological matrices by high performance liquid chromatography electrospray ionization mass spectrometry.

@article{McDonald2007ExtractionAA,
  title={Extraction and analysis of sterols in biological matrices by high performance liquid chromatography electrospray ionization mass spectrometry.},
  author={J. McDonald and Bonne M. Thompson and Erin C McCrum and D. Russell},
  journal={Methods in enzymology},
  year={2007},
  volume={432},
  pages={
          145-70
        }
}
  • J. McDonald, Bonne M. Thompson, +1 author D. Russell
  • Published 2007
  • Chemistry, Medicine
  • Methods in enzymology
  • We describe the development of a high performance liquid chromatography mass spectrometry (HPLC-MS) method that allows the identification and quantitation of sterols in mammalian cells and tissues. Bulk lipids are extracted from biological samples by a modified Bligh/Dyer procedure in the presence of eight deuterated sterol standards to allow subsequent quantitation and determination of extraction efficiency. Sterols and other lipids are resolved by HPLC on a reverse-phase C18 column using a… CONTINUE READING
    113 Citations
    Highly sensitive and specific analysis of sterol profiles in biological samples by HPLC–ESI–MS/MS
    • 42
    Direct analysis of sterols by derivatization matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and tandem mass spectrometry.
    • 22
    Liquid chromatography-tandem mass spectrometry determination of plasma 24S-hydroxycholesterol with chromatographic separation of 25-hydroxycholesterol.
    • 41
    • Highly Influenced

    References

    SHOWING 1-10 OF 25 REFERENCES
    Quantitation of receptor ligands by mass spectrometry.
    • 15
    Inhibitors of sterol synthesis. Chromatography of acetate derivatives of oxygenated sterols.
    • 78
    • PDF
    Chromatographic analysis of plant sterols in foods and vegetable oils.
    • S. Abidi
    • Chemistry, Medicine
    • Journal of chromatography. A
    • 2001
    • 253
    A rapid method of total lipid extraction and purification.
    • E. Bligh, W. Dyer
    • Biology, Medicine
    • Canadian journal of biochemistry and physiology
    • 1959
    • 42,369
    • PDF