Extraction and analysis of auxins in plants using dispersive liquid-liquid microextraction followed by high-performance liquid chromatography with fluorescence detection.

@article{Lu2010ExtractionAA,
  title={Extraction and analysis of auxins in plants using dispersive liquid-liquid microextraction followed by high-performance liquid chromatography with fluorescence detection.},
  author={Qiaomei Lu and Lihui Chen and Minghua Lu and Guonan Chen and Lan Zhang},
  journal={Journal of agricultural and food chemistry},
  year={2010},
  volume={58 5},
  pages={2763-70}
}
Auxin plays an important role in cell differentiation, apical dominance, and tropism in plants. A new method based on dispersive liquid-liquid microextraction (DLLME) combined with high-performance liquid chromatography-fluorescence detection (HPLC-FLD) has been established to detect auxin. A mixture of CHCl(3) (extraction solvent) and acetone (disperser solvent) was injected quickly into a sample solution with desired salt concentration and pH value, and then a cloudy solution consisting of… CONTINUE READING

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