Extracellular vesicle isolation and characterization: toward clinical application.

  title={Extracellular vesicle isolation and characterization: toward clinical application.},
  author={Rong Xu and David W. Greening and Hong-jian Zhu and Nobuhiro Takahashi and Richard J. Simpson},
  journal={The Journal of clinical investigation},
  volume={126 4},
Two broad categories of extracellular vesicles (EVs), exosomes and shed microvesicles (sMVs), which differ in size distribution as well as protein and RNA profiles, have been described. EVs are known to play key roles in cell-cell communication, acting proximally as well as systemically. This Review discusses the nature of EV subtypes, strategies for isolating EVs from both cell-culture media and body fluids, and procedures for quantifying EVs. We also discuss proteins selectively enriched in… CONTINUE READING

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