Extracellular sulfite is protective against reactive oxygen species and antibiotic stress in Shewanella oneidensis MR-1.

@article{Goff2021ExtracellularSI,
  title={Extracellular sulfite is protective against reactive oxygen species and antibiotic stress in Shewanella oneidensis MR-1.},
  author={Jennifer L. Goff and Jeffra K. Schaefer and Nathan W. Yee},
  journal={Environmental microbiology reports},
  year={2021}
}
In this study, we investigated the extracellular reactive sulfur species produced by Shewanella oneidensis MR-1 during growth. The results showed that sulfite is the major extracellular sulfur metabolite released to the growth medium under both aerobic and anaerobic growth conditions. Exogenous sulfite at physiological concentrations protected S. oneidensis MR-1 from hydrogen peroxide toxicity and enhanced tolerance to the beta-lactam antibiotics cefazolin, meropenem, doripenem and ertapenem… 

References

SHOWING 1-10 OF 27 REFERENCES

H2S: A Universal Defense Against Antibiotics in Bacteria

It is demonstrated that inactivation of putative cystathionine β-synthase, cystATHionine γ-lyase, or 3-mercaptopyruvate sulfurtransferase in Bacillus anthracis, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli suppresses H2S production, rendering these pathogens highly sensitive to a multitude of antibiotics.

Mechanism of H2S-mediated protection against oxidative stress in Escherichia coli

It is reported that 3-mercaptopyruvate sulfurtransferase (3MST) is the major source of endogenous H2S in Escherichia coli and 3MST protects E. coli against oxidative stress via l-cysteine utilization and H 2S-mediated sequestration of free iron necessary for the genotoxic Fenton reaction.

Endogenous generation of hydrogen sulfide and its regulation in Shewanella oneidensis

A screen for regulators controlling endogenous H2S generation by transposon mutagenesis identified global regulator Crp to be essential to all H 2S-generating processes and Fnr and Arc, two other global regulators that have a role in respiration, are dispensable in regulating H1S generation via respiration of sulfur species.

Role of extracellular reactive sulfur metabolites on microbial Se(0) dissolution

The findings suggest that the production of reactive sulfur metabolites by soil microorganisms and the formation of soluble selenosulfur complexes can promote selenium mobilization during chemical weathering.

The octahaem SirA catalyses dissimilatory sulfite reduction in Shewanella oneidensis MR-1.

Analysis of MR-1 cultures grown anaerobically with sulfite suggested that the dissimilatory sulfite reductase catalyses six-electron reduction of sulfite to sulfide.

Breathing Iron: Molecular Mechanism of Microbial Iron Reduction by Shewanella oneidensis

The chapter highlights the mechanistic details associated with each of the four Fe(III) reduction pathways of S. oneidensis, including a concluding discussion of the future research directions for each pathway.

Phylogeny of sulfate‐reducing bacteria

Physiological characteristics particular to each of these groups are discussed, as is the availability of tested group-specific phylogenetic probes and PCR primers directed toward individual groups.

The DUF81 protein TauE in Cupriavidus necator H16, a sulfite exporter in the metabolism of C2 sulfonates.

RT-PCR showed inducible transcription of these three genes, and led to the hypothesis that H16_B1872 and orthologous proteins represent a sulfite exporter, which was named TauE.

Respiration-linked proton translocation coupled to anaerobic reduction of manganese(IV) and iron(III) in Shewanella putrefaciens MR-1

An oxidant pulse technique, with lactate as the electron donor, was used to study respiration-linked proton translocation in the manganese- and iron-reducing bacterium Shewanella putrefaciens MR-1.

Sulfur metabolism in Escherichia coli and related bacteria: facts and fiction.

The situation in Escherichia coli and related bacteria, where more than one hundred genes involved in sulfur metabolism have already been discovered, is reviewed, and the involvement of methionine as the universal start of proteins as well as its derivative S-adenosylmethionine in a vast variety of cell processes argue in favour of a major importance of sulfur metabolism in all organisms.