Extracellular stress and lipopolysaccharide modulate Acinetobacter baumannii surface-associated motility

@article{McQueary2012ExtracellularSA,
  title={Extracellular stress and lipopolysaccharide modulate Acinetobacter baumannii surface-associated motility},
  author={Christin N. McQueary and Benjamin Kirkup and Yuanzheng Si and Miriam Barlow and Luis A. Actis and David W. Craft and Daniel V. Zurawski},
  journal={Journal of Microbiology},
  year={2012},
  volume={50},
  pages={434-443}
}
Acinetobacter baumannii is a nosocomial bacterial pathogen, and infections attributed to this species are further complicated by a remarkable ability to acquire antimicrobial resistance genes and to survive in a desiccated state. While the antibiotic resistance and biofilm formation of A. baumannii is well-documented, less is known about the virulence attributes of this organism. Recent studies reported A. baumannii strains display a motility phenotype, which appears to be partially dependent… 
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TLDR
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TLDR
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References

SHOWING 1-10 OF 94 REFERENCES
Acinetobacter baumannii Increases Tolerance to Antibiotics in Response to Monovalent Cations
TLDR
NaCl is identified as being an important signal that induces significant tolerance to aminoglycosides, carbapenems, quinolones, and colistin upon the culturing of A. baumannii cells in physiological NaCl concentrations, demonstrating broad-spectrum tolerance to antibiotics.
Genetic analysis of surface motility in Acinetobacter baumannii.
TLDR
Transposon mutagenesis was used to identify additional genes required for motility and revealed loci encoding various functions: non-ribosomal synthesis of a putative lipopeptide, a sensor kinase (BfmS), a lytic transglycosylase, O-antigen biosynthesis, an outer membrane porin (OmpA) and de novo purine biosynthesis (PurK).
The K1 Capsular Polysaccharide of Acinetobacter baumannii Strain 307-0294 Is a Major Virulence Factor
TLDR
The results from this study are the first to address the role of capsule in the pathogenesis of A. baumannii infection and suggested that conserved proteins, which contribute to the capsule-positive phenotype, are potential antivirulence drug targets.
Acinetobacter baumannii biofilms: Variations among strains and correlations with other cell properties
TLDR
Although the ATCC 19606T type strain and 8 different clinical isolates form biofilms, there are significant variations in the cell density and microscopic structures of these cell aggregates, with 3 of the isolates forming pellicles floating on the surface of stagnant broth cultures.
Inactivation of Phospholipase D Diminishes Acinetobacter baumannii Pathogenesis
TLDR
Results suggest that PLD is an A. baumannii virulence factor that causes a reduction in the organism's ability to thrive in serum, a deficiency in epithelial cell invasion, and diminished pathogenesis in a murine model of pneumonia.
The Opportunistic Human Pathogen Acinetobacter baumannii Senses and Responds to Light
TLDR
It is shown that the opportunistic pathogen Acinetobacter baumannii senses and responds to blue light, and the A1S_2225 gene, which codes for an 18.6-kDa protein that contains an N-terminal blue-light-sensing-using flavin (BLUF) domain and lacks a detectable output domain(s), was named blue-lightsensing A (blsA).
Iron acquisition functions expressed by the human pathogen Acinetobacter baumannii
TLDR
Gene mapping and protein analysis together with complementation assays showed that a protein related to SecA, which is a component of the Sec protein secretion system in a wide range of bacteria, is needed at least for the production of the BauA acinetobactin outer membrane receptor.
The Acinetobacter baumannii 19606 OmpA Protein Plays a Role in Biofilm Formation on Abiotic Surfaces and in the Interaction of This Pathogen with Eukaryotic Cells
TLDR
It is shown that although outer membrane protein A (OmpA) of A. baumannii 19606 plays a partial role in the development of robust biofilms on plastic, it is essential for bacterial attachment to Candida albicans filaments and A549 human alveolar epithelial cells.
Identification and Characterization of an Acinetobacter baumannii Biofilm-Associated Protein
TLDR
This is the first identification of a specific cell surface protein directly involved in biofilm formation by A. baumannii and suggests that Bap is involved in intercellular adhesion within the mature biofilm.
Attachment to and biofilm formation on abiotic surfaces by Acinetobacter baumannii: involvement of a novel chaperone-usher pili assembly system.
TLDR
Results demonstrate that the expression of a chaperone-usher secretion system, some of whose components appear to be acquired from unrelated sources, is required for pili formation and the concomitant attachment to plastic surfaces and the ensuing formation of biofilms by A. baumannii cells.
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