Extracellular proteinase and chitinase produced by a culture ofStreptomyces kurssanovii

  title={Extracellular proteinase and chitinase produced by a culture ofStreptomyces kurssanovii},
  author={Alla V. Il’ina and Natalya Yu. Tatarinova and Vladimir E. Tikhonov and Valery P. Varlamov},
  journal={Applied Biochemistry and Microbiology},
Extracellular enzymes—a chitinase and a proteinase with molecular weights 22 and 32 kDa, respectively—were isolated fromStreptomyces kurssanovii cells. After purification on modified regenerated chitin, the enzymes were virtually homogeneous according to denaturing PAGE. Both enzymes were found to degrade chitosan. 


Preparation of affinity sorbents and isolation of individual chitinases from a crude supernatant produced by Streptomyces kurssanovii by a one-step affinity-chromatographic system.
The preparation of two affinity-chromatography sorbents based on cross-linked chitin that retained selectively one of the four extracellular chitInases in the culture supernatant produced by Streptomyces kurssanovii were shown to be stable over the period of separation and could be used repeatedly.
Purification and mode of action of a chitosanase from Penicillium islandicum
Both residues were needed for cleavage, and polymers containing equal proportions of acetylated and non-acetylated sugars were optimal for chitosanase activity, which strongly indicated that P. islandicum chitOSanase cleaved chitan between N- acetylglucosamine and glucosamine.
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four major
Investigation of the hydrodynamic properties of chitosan solutions
The hydrodynamic properties of solutions of polymer-homologous of chitosan are studied. The solvents used are 2% acetic acid with 0.2 M sodium acetate and dichloroacetic acid of equal ionic strength.
Selective N-acylation of chitosan.