Extracellular nucleotides mediate Ca2+ fluxes in J774 macrophages by two distinct mechanisms.

@article{Greenberg1988ExtracellularNM,
  title={Extracellular nucleotides mediate Ca2+ fluxes in J774 macrophages by two distinct mechanisms.},
  author={Steven A. Greenberg and Francesco Di Virgilio and Thomas H. Steinberg and Samuel C. Silverstein},
  journal={The Journal of biological chemistry},
  year={1988},
  volume={263 21},
  pages={10337-43}
}
We have studied the effects of extracellular nucleotides on the cytosolic free calcium concentration [( Ca2+]i) in J774 macrophages using quin2 and indo-1 as indicator dyes. Micromolar quantities of ATP induced a biphasic increase in [Ca2+]i: a rapid and transient increase (peak I) which was due to mobilization of Ca2+ from intracellular stores and a second more sustained elevation (peak II) due to influx of extracellular Ca2+. The sustained peak II elevation had two components, a "low… CONTINUE READING